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KMID : 0381120080300060515
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Genes and Genomics
2008 Volume.30 No. 6 p.515 ~ p.522
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Isolation of Eight DM domains of Dmrt genes in Rana livida (Amphibia: Anura)
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Jia Rui
Nie Liu-Wang
Wang Jing-Jing
Wang Ning
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Abstract
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The Dmrt gene family encodes putative transcription factors, sharing a highly conserved DNA-binding domain?the DM domain, related to the sexual regulators Doublesex from Drosophila melanogaster and MAB-3 from Caenorhabditis elegans. Besides sex determination or differentiation, this gene family has evolved to adopt functions in other developmental pathways. In this paper, eight distinct DM related transcription factor (Dmrt) genes from both male and female and detected using PCR and the SSCP technique. No sexual differences were observed. These fragments (named RlDmrt1a, RlDmrt1b, RlDmrt2a, RlDmrt2b, RlDmrt2c, RlDmrt3, RlDmrt4, RlDmrt5) exhibited 98%, 98%, 95%, 95%, 95%, 99%, 92% and 83% similarity respectively to the corresponding homologous human Dmrt genes. Phylogenetic analysis based on DM domain amino acid sequence similarities suggested that vertebrate and invertebrate Dmrt genes can be grouped into seven distinct subfamilies. Interestingly, sequence analysis further illustrates that the gene Dmrt1 and 2 found in R. livida are duplicated. The DM domains of R. livida genes contained two conserved zinc-chelating sites (CCHC and HCCC). Identification of these genes is a potential step in understanding development regulations including sex determination in green cascade frog.
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KEYWORD
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Rana livida, Dmrt genes, SSCP, Phylogenetic analysis
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